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collagen coated coverslips  (Thermo Fisher)


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    Structured Review

    Thermo Fisher collagen coated coverslips
    Collagen Coated Coverslips, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+coated+coverslips/Collagen/pmc13018973-248-23-25
    Average 99 stars, based on 1 article reviews
    collagen coated coverslips - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Immunofluorescence:


    Incubation:


    Article Title: Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains
    Article Snippet: The next day, membranes were washed with TBS-T (0.1% Tween-20) and incubated with secondary antibodies (Li-Cor, IRDye 680/800, 1:10000) for 1h at room temperature (~23°C±2°C) prior to TBS-T washes and imaging (Odyssey CLx imaging system). .. For immunofluorescent characterization of optoFUS and ssTDP-43 inclusions, cells seeded onto collagen-coated coverslips (Thermo Fisher, 50μg/mL) were first fixed for 15min at room temperature (~23°C±2°C) in 4% PFA following one PBS wash. Three additional PBS washes were then performed prior to a 1h incubation in blocking buffer (0.3% TX-100/5% NDS in PBS) at room temperature (~23°C±2°C). .. Cells were then incubated overnight at 4°C with primary antibodies diluted in blocking buffer at the following concentrations: rabbit anti-TAF15/TAFII68 (Bethyl Labs, 1:500), mouse anti-EWSR1 (Santa Cruz, 1:200), rat anti-methylated TLS/FUS (Clone 9G6, Sigma-Aldrich, 1:100), guinea pig anti-MAP2 (Synaptic Systems, 1:1000), rabbit anti-G3BP1 (Proteintech, 1:500), rat anti-phospho-TDP43 (S409/410) (Clone 1D3, Biolegend, 1:200), rabbit anti-SQSTM1/p62 (Abcam, 1:500).

    Blocking Assay:


    Article Title: Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains
    Article Snippet: The next day, membranes were washed with TBS-T (0.1% Tween-20) and incubated with secondary antibodies (Li-Cor, IRDye 680/800, 1:10000) for 1h at room temperature (~23°C±2°C) prior to TBS-T washes and imaging (Odyssey CLx imaging system). .. For immunofluorescent characterization of optoFUS and ssTDP-43 inclusions, cells seeded onto collagen-coated coverslips (Thermo Fisher, 50μg/mL) were first fixed for 15min at room temperature (~23°C±2°C) in 4% PFA following one PBS wash. Three additional PBS washes were then performed prior to a 1h incubation in blocking buffer (0.3% TX-100/5% NDS in PBS) at room temperature (~23°C±2°C). .. Cells were then incubated overnight at 4°C with primary antibodies diluted in blocking buffer at the following concentrations: rabbit anti-TAF15/TAFII68 (Bethyl Labs, 1:500), mouse anti-EWSR1 (Santa Cruz, 1:200), rat anti-methylated TLS/FUS (Clone 9G6, Sigma-Aldrich, 1:100), guinea pig anti-MAP2 (Synaptic Systems, 1:1000), rabbit anti-G3BP1 (Proteintech, 1:500), rat anti-phospho-TDP43 (S409/410) (Clone 1D3, Biolegend, 1:200), rabbit anti-SQSTM1/p62 (Abcam, 1:500).

    Imaging:

    Article Title: TRPV6 channel function is involved in endometrial epithelial cell Ca 2+ signaling and female mouse fecundity.
    Article Snippet: .. Ca2+ imaging in isolated MEECs MEECs from Trpv6−/−, Trpv6mt/mt and wild-type mice, plated on 25 mm collagen-coated coverslips, were loaded with 5 μM Fura-2 AM (Invitrogen) for 30 min at RT in Ringer’s solution containing 115 mM NaCl, 5 mM KCl, 2 mM CaCl2, 2 mM MgCl2, 10 mM HEPES, 10 mM glucose, pH 7.4. .. Subsequently, the coverslips were placed in a bath chamber, washed three times with Ringer ́s solution and mounted with a volume of 300 μL of nominal Ca2+free or Ca2+-containing Ringer ́s solution (see start of experiments in Fig. 4, with or without ORAI channel blocker GSK7975A or BTP2 (both Merck)) on the stage of a Zeiss AxioVert S100 inverted microscope equipped with a Fluar-20x/0.75 objective (Zeiss), a monochromator (Polychrom V, TILL Photonics) and a charge-coupled device camera (Clara CCD, Andor Technology).

    Isolation:

    Article Title: TRPV6 channel function is involved in endometrial epithelial cell Ca 2+ signaling and female mouse fecundity.
    Article Snippet: .. Ca2+ imaging in isolated MEECs MEECs from Trpv6−/−, Trpv6mt/mt and wild-type mice, plated on 25 mm collagen-coated coverslips, were loaded with 5 μM Fura-2 AM (Invitrogen) for 30 min at RT in Ringer’s solution containing 115 mM NaCl, 5 mM KCl, 2 mM CaCl2, 2 mM MgCl2, 10 mM HEPES, 10 mM glucose, pH 7.4. .. Subsequently, the coverslips were placed in a bath chamber, washed three times with Ringer ́s solution and mounted with a volume of 300 μL of nominal Ca2+free or Ca2+-containing Ringer ́s solution (see start of experiments in Fig. 4, with or without ORAI channel blocker GSK7975A or BTP2 (both Merck)) on the stage of a Zeiss AxioVert S100 inverted microscope equipped with a Fluar-20x/0.75 objective (Zeiss), a monochromator (Polychrom V, TILL Photonics) and a charge-coupled device camera (Clara CCD, Andor Technology).

    TUNEL Assay:

    Article Title: Tryptophan metabolites 3-hydroxykynurenine (3HK) and 3-hydroxyanthranilic acid (3HAA) increase oxidative stress and impair osteoblastic bone formation.
    Article Snippet: The absorbance was read via 566 an Agilent Biotek Cytation 5 multi-mode microplate reader at 590 nm, and cell viability was 567 calculated relative to the vehicle condition. .. 568 569 4.5 TUNEL assay 570 After determining the 50% inhibitory concentration (IC50) of 3HK and 3HAA via MTT 571 assays, ST2 and C2C12 cells were seeded on collagen-coated coverslips (ThermoFisher 572 #A1048301) and allowed to attach in a humidified CO2 incubator. ..

    Concentration Assay:

    Article Title: Tryptophan metabolites 3-hydroxykynurenine (3HK) and 3-hydroxyanthranilic acid (3HAA) increase oxidative stress and impair osteoblastic bone formation.
    Article Snippet: The absorbance was read via 566 an Agilent Biotek Cytation 5 multi-mode microplate reader at 590 nm, and cell viability was 567 calculated relative to the vehicle condition. .. 568 569 4.5 TUNEL assay 570 After determining the 50% inhibitory concentration (IC50) of 3HK and 3HAA via MTT 571 assays, ST2 and C2C12 cells were seeded on collagen-coated coverslips (ThermoFisher 572 #A1048301) and allowed to attach in a humidified CO2 incubator. ..

    Article Title: Tryptophan metabolites 3-hydroxykynurenine (3HK) and 3-hydroxyanthranilic acid (3HAA) increase oxidative stress and impair osteoblastic bone formation
    Article Snippet: The absorbance was read via an Agilent Biotek Cytation 5 multi-mode microplate reader at 590 nm, and cell viability was calculated relative to the vehicle condition. .. After determining the 50% inhibitory concentration (IC 50 ) of 3HK and 3HAA via MTT assays, ST2 and C2C12 cells were seeded on collagen-coated coverslips (Thermo Fisher Scientific #A1048301) and allowed to attach in a humidified CO 2 incubator. ..

    MTT Assay:

    Article Title: Tryptophan metabolites 3-hydroxykynurenine (3HK) and 3-hydroxyanthranilic acid (3HAA) increase oxidative stress and impair osteoblastic bone formation.
    Article Snippet: The absorbance was read via 566 an Agilent Biotek Cytation 5 multi-mode microplate reader at 590 nm, and cell viability was 567 calculated relative to the vehicle condition. .. 568 569 4.5 TUNEL assay 570 After determining the 50% inhibitory concentration (IC50) of 3HK and 3HAA via MTT 571 assays, ST2 and C2C12 cells were seeded on collagen-coated coverslips (ThermoFisher 572 #A1048301) and allowed to attach in a humidified CO2 incubator. ..

    Article Title: Tryptophan metabolites 3-hydroxykynurenine (3HK) and 3-hydroxyanthranilic acid (3HAA) increase oxidative stress and impair osteoblastic bone formation
    Article Snippet: The absorbance was read via an Agilent Biotek Cytation 5 multi-mode microplate reader at 590 nm, and cell viability was calculated relative to the vehicle condition. .. After determining the 50% inhibitory concentration (IC 50 ) of 3HK and 3HAA via MTT assays, ST2 and C2C12 cells were seeded on collagen-coated coverslips (Thermo Fisher Scientific #A1048301) and allowed to attach in a humidified CO 2 incubator. ..



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